Table S2.

Sequences of in vitro transcribed RNA and DNA oligos used for in vitro transcription

Name	Sequence
Transcribed RNA
MHV	GUAUAAGAGUGAUUGGCGUCCGUACGUACCCUCUCAACUCU
HCoV	ACUUAAGUACCUUAUCUAUCUACAGAUAGAAAAGUUGCUUUU
GGG42	GGGUAAGUACCUUAUCUAUCUACAGAUAGAAAAGUUGCUUUU
ss44	GGGAGAGAGAGAGAGAGAGUAAGGGCGUCGUCGCCCCGAGAAUU
43*	GGGUAAGUACCUUAUCUAUCUACAGAUAGAAAAGUUGCUAAAA
Reporter 5′-UTR†	GAAUACACGGAAUUCAGCAAUUCGAGCUCGCCCGGGGAUCUGCGAUCUAAGUAAGCUUGGCAUU CCGGUACUGUUGGUAAAGCCACCAUG
DNA Oligo templates (5′ → 3′)
T7‡	TAATACGACTCACTATAG
T7Φ2.5§	TAATACGACTCACTATTA
MHV	AGAGTTGAGAGGGTACGTACGGACGCCAATCACTCTTATACTATAGTGAGTCGTATTA
HCoV	AAAAGCAACTTTTCTATCTGTAGATAGATAAGGTACTTAAGTAATAGTGAGTCGTATTA
GGG42	AAAAGCAACTTTTCTATCTGTAGATAGATAAGGTACTTACCCTATAGTGAGTCGTATTA
ss44	AATTCTCGGGGCGACGACGCCCTTACTCTCTCTCTCTCTCTCCCTATAGTGAGTCGTATTA
43	TTTTAGCAACTTTTCTATCTGTAGATAGATAAGGTACTTACCCTATAGTGAGTCGTATTA

^*This RNA is similar to GGG42, except that it is designed to have a 5-nt overhang to allow strong IFIT1 binding, and a free 3′-end with sequence AAA to improve pCp-Cy5 labeling efficiency with T4 RNA ligase.

^†Start codon underlined, this transcript was prepared with a plasmid and not a DNA oligo.

^‡Promoter top strand for all sequences except HCoV and mRNA reporter.

^§Promoter top strand for HCoV.