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Efficiencies of various plastidial thioredoxins in the activation of a mutant NADP-MDH where only the N-terminal disulfide is left (mutant C207A/C365A/C377A). Mutant NADP-MDH was activated in the presence of either 10 mm DTT (⋄), or DTT plus 20 μm Trxs f1 (□), or m1 (○), or y1 (▿), or y2 (▾). Aliquots (5 μg of enzyme) were withdrawn periodically for activity measurements.
