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Activation kinetics of wild-type sorghum NADP-MDH with 1 μm Trx f1, with or without 20 μm Trx y1. Trx y1 alone (▿); Trx f1 alone (•); Trx f1 and Trx y1 added at the beginning of activation (▾); preincubation with DTT-reduced 20 μm Trx y1 for 10 min, then addition of 1 μm Trx f1 (○). Aliquots (5 μg of enzyme) were withdrawn periodically for activity measurements.
