Fig. S1.
Flipped SNAP25 and SNAP23, but not SNAP29, promote cell surface expression of flipped STX11 constructs. (A) MEF 3T3 cells were transiently cotransfected with either STX11–PDGF, Syx11H3–PDGF, STX11–GPI, or STX11H3–GPI and with flipped SNAP25, -23, -29, or vector alone. Cells were fixed and incubated in the presence (permeab.) or absence (nonpermeab) of 0.1% Triton X-100. Cells were stained with anti–c-myc antibody and images were taken using confocal microscopy. (B) Cell surface quantification of different flipped STX11 constructs. Total fluorescent intensity per cell in nonpermeabilized cells stained with anti–c-myc antibody as shown in A were quantified in 75 cells for each condition. Results are mean ± SD of three independent experiments.