Fig. S3.

Munc18-2 promotes transition from incomplete to complete fusion by acting on partially assembled SNARE complex. (A) Schematic representation of order of addition experiments using the cell fusion assay as described in Fig. 1A in which Munc18-2^WT was added at different times as indicated. Prefusion condition, Munc18-2^WT was added at the time the v- ant t- cells were mix (Pre); Postfusion, Munc18-2^WT was added after 10 h of the v ant t cells were mix (post); Munc18-2^WT (partially assembled) or buffer (control) were added at the time of mixing v ant t cells, incubated for 6 h and cdV8 was added for an additional 6 h. (B) Graph showing the percentage of cells that underwent complete fusion (black bars) and lipid mixing (striped bars) in the cell fusion experiments described above in A.