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. 2017 Mar 6;2017:4828936. doi: 10.1155/2017/4828936

Figure 2.

Figure 2

Secreted ISGs restrict early steps of HBV infection. (a) Differentiated HepaRG cells were treated with 1000 IU/mL of IFN-α. One day later, the medium was removed. Cells were washed three times with PBS and refilled with new medium. The resulting medium containing interferon induced factors (ISG+) was collected 48 hours later and then mixed with HBV and PEG. The mixture then transferred onto fresh differentiated HepaRG cell for HBV infection. (b) HBV cccDNA and intracellular DNA were evaluated by qPCR 4 days after infection. (c) Differentiated HepaRG cells were infected with HBV (MOI = 200) and 7 days later different treatments were applied as indicated. Intracellular HBV-DNA was measured 4 days after treatment. (d) Differentiated HepaRG cells were incubated with indicated treatment for 24 hours and then infected with HBV (MOI = 200). Intracellular HBV-DNA was measured 4 days after infection. Data are means ± s.d. ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001 by Student's unpaired two-tailed t-test.