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. 2017 Mar 23;2(6):e83527. doi: 10.1172/jci.insight.83527

Figure 2. Neutralizing antibodies against CHIKV vaccine.

Figure 2

Animals were immunized on days 0 and/or 42 (y axis and black vertical dotted line). Animals were bled on days 0, 14, 34, 56, 77, 98, 112, 123, 127, 140, 154, 182, 210, and 298 (end of followup), and antibody levels in serum were determined. (A) Δ5 virus (red, 1×) corresponding to group Δ5-A (see Figure 1B), (B) DD (green), (C) DM (blue), (D) saline (orange). Animals were challenged on day 123 (red vertical dotted line). Panels A–D show antibody levels in individual animals. Panel E compares the geometric mean titers obtained for animals in panels A–D. Panel F shows NT titer of sera against Caribbean (CB, filled boxes) CHIKV isolate compared with response against East/Central/South African (ECSA) strain from day 56 (14 days after Δ5 or after D or M boost, respectively) for the vaccinated sera (panels A–C) or from day 140 (14 days after challenge with WT CHIKV) for the vaccine control animals (D). Sera from control animals were sampled after challenge with WT CHIKV. Statistical analyses were performed using the Kruskal-Wallis test followed by a 2-tailed Mann-Whitney U test to analyze differences between two.