Table I.
Primers used to generate the HvPht1;1 promoter deletion series and modified promoters
| Promoter/Fragment | Primer ID | Primer Sequence |
|---|---|---|
| HvPht1;1 (full-length) | Pht1;1 5′ (forward) | ctcactagaGCGGCCGCTTAATTAAGAGCTCCGACTACCCCCG |
| Pht1;1-Δ1 | −1151 5′ (forward) | ctcactagaTTAATTAACAAAATGCAATCAACCGAC |
| Pht1;1-Δ2 | −856 5′ (forward) | ctcactagaTTAATTAACTCGTAAACTGATAGCACG |
| Pht1;1-Δ3 | −546 5′ (forward) | ctcactagaTTAATTAAGTTGCTAAGTTTGACATAGC |
| Pht1;1-Δ4 | −333 5′ (forward) | ctcactagaTTAATTAAGCTCAATCCTTGCGATAAC |
| Pht1;1-Δ5 | −195 5′ (forward) | ctcactagaTTAATTAATGTACGTACGTGCAATGC |
| HvPht1;1, plus Δ1-Δ5 | Pht1;1 3′ (reverse) | gatccagtgGGCGCGCCGGTCGCCGGCGATCTC |
| 3′end Δ | −1151 5′ (forward | (as above) |
| −86Δ 3′ (reverse) | gatccagtgGGCGCGCCGTAGTACGTATATATAGGCA | |
| Δ2f | −856 5′ (forward) | (as above) |
| LinkΔ5-Δ2 (reverse) | tacggcattgcaCGTACGTACATGTAAACGAGAGAAAATAATGAG | |
| Δ2fmut | −856 5′ (forward) | (as above) |
| LinkΔ5-Δ2mut (reverse) | tacggcattgcaCGTACGTACATGTAAACGAGAGAAAATAATGAGAAGATCCGACTCTGCCTGAG | |
| Δ5 | LinkΔ2-Δ5 (forward) | cgtttacatgtaCGTACGTGCAATGC |
| Pht1;1 3′ (reverse) | (as above) | |
| Δ5mut | LinkΔ2-Δ5mut (forward) | cgtttacatgtaCGTACGTGCAATGCCGTACATGGAGATTTTAATATTTACAAGTAGCGAGGAAATGTCCCTTTGGCAGAGCCGCCGAAC |
| Pht1;1 3′ (reverse) | (as above) | |
| OGD3 | LinkΔ2-OGD3 (forward) | cgtttacatgtaCGTACGCATCATGTTTATCCTGGAC |
| OGD3 3′ (reverse) | gatccagtgGGCGCGCCTGTTTATTGCTCTTGCTTGC |
The mutated P1BS-like motifs (GnAGAGnC) contained within the linkΔ5-Δ2mut (reverse) primer and the linkΔ2-Δ5mut (forward) primer are shown in bold. Incorporated endonuclease restriction site sequences are underlined. 5Δ Spacer sequences are indicated in lowercase.