Figure 2.
Production of ent-CPP. A, GC-MS analysis (275 m/z extracted ion chromatographs) of the dephosphorylated reaction products from GGPP produced by the indicated enzyme. B, Mass spectrum of the GC-MS 275 m/z chromatograph peak for OsCPS1ent (RT = 14.12 min); C, that for OsCPS2ent (RT = 14.12); and D, that for authentic dephosphorylated ent-CPP (i.e. ent-copalol), which also exhibits RT = 14.12 min and is clearly separable from dephosphorylated syn-CPP (i.e. syn-copalol; RT = 13.96 min) and GGPP (i.e. geranylgeraniol; RT = 13.87 min).