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. 2017 Mar 23;2(6):e89206. doi: 10.1172/jci.insight.89206

Figure 2. Systemic in vivo ibuprofen treatment during weaning-induced mammary gland involution decreases mammary fibroblast activation.

Figure 2

(A) Schematic shows breeding and weaning-induced mammary gland involution timeline with 0 mg/kg (control), 150 mg/kg (150IBU), and 300 mg/kg (300IBU) ibuprofen provided in chow for 6 days following weaning, with mammary fibroblasts FACS-isolated on involution day 6 (InvD6). (B) Representative H&E images of nulliparous, InvD6, and 300IBU mammary glands depicting normal nulliparous and InvD6 gland morphology in the absence and presence of ibuprofen treatment. Scale bar: 200 μm. (C) Left: RT-qPCR of Col1a1 gene expression in isolated fibroblasts, n = 4–7 per group. Right: type I collagen IHC quantification around InvD5 mammary lobules (InvD5T) with and without 300IBU treatment for 5 days, n = 5–6 per group. (D) RT-qPCR analysis of targeted genes in isolated fibroblasts, n = 4–7 per group. Gene expression data are normalized to Actb. Ibuprofen intervention data are from 2 independent breeding studies. *P < 0.05 by 1-way ANOVA with Tukey correction and unpaired 2-tailed t test. Data represent mean ± SEM.