Figure 3.
16:3Δ7,10,13 content in leaves of Arabidopsis fad5 mutant plants expressing genomic DNA containing At3g15850. A genomic ADS3 clone was expressed in fad5 mutant plants, and fatty acid patterns from 1-week-old second-generation leaves were analyzed by gas chromatography/mass spectrometry. A, Wild type; B, fad5; C, fad5 line expressing the genomic ADS3 fragment under its endogenous promoter. Arrows, Elution of 16:3Δ7,10,13. Numbers denote 16:3Δ7,10,13 quantifications as mol% of total fatty acids. Elution of other fatty acids as indicated.