Table I.
Fatty acid composition in leaves of wild-type Arabidopsis, fad5 mutants, fad5 mutants expressing either genomic DNA containing ADS3 or ADS cDNA constructs, and SALK_142406, a T-DNA insertion mutant of ADS3.2
| Fatty Acid
|
Wild Type
|
fad5
|
Genomic ADS3
|
cDNA
|
SALK_142406
|
||||
|---|---|---|---|---|---|---|---|---|---|
| Line C1 | Line C10 | Line C T3 | ADS31-71-ADS1 | ADS31-71-ADS2 | ADS3 | ||||
| 16:0 | 14.1 ± 2.2 | 19.6 ± 1.8 | 15.2 ± 2.0 | 14.2 ± 1.5 | 14.6 ± 1.8 | 16.6 ± 2.1 | 17.0 ± 2.1 | 16.4 ± 2.4 | 13.8 ± 2.0 |
| 16:1 | 2.7 ± 0.5 | 3.1 ± 0.8 | 2.9 ± 0.5 | 3.1 ± 0.6 | 2.6 ± 0.5 | 2.1 ± 0.3 | 2.0 ± 1.0 | 2.8 ± 1.3 | 2.9 ± 0.5 |
| 16:3 | 12.1 ± 1.6 | n.d. | 11.2 ± 1.1 | 10.0 ± 1.2 | 12.4 ± 1.1 | 4.5 ± 1.2 | 3.5 ± 0.9 | 6.6 ± 2.3 | 12.3 ± 1.7 |
| 18:0 | 1.5 ± 0.2 | 2.0 ± 0.4 | 2.8 ± 0.3 | 3.1 ± 0.4 | 1.8 ± 0.6 | 6.3 ± 0.9 | 4.1 ± 0.5 | 3.3 ± 1.0 | 1.8 ± 0.5 |
| 18:1 | 5.6 ± 0.2 | 6.2 ± 0.4 | 5.9 ± 0.4 | 4.9 ± 0.3 | 3.7 ± 0.7 | 6.6 ± 0.2 | 5.5 ± 1.2 | 5.8 ± 0.9 | 4.9 ± 0.6 |
| 18:2 | 17.0 ± 2.0 | 20.3 ± 1.6 | 17.5 ± 1.3 | 16.3 ± 2.1 | 15.8 ± 1.5 | 15.2 ± 2.2 | 17.3 ± 3.5 | 17.7 ± 2.9 | 16.2 ± 2.5 |
| 18:3 | 46.4 ± 3.4 | 49.2 ± 2.6 | 43.2 ± 2.1 | 47.8 ± 2.5 | 48.1 ± 2.6 | 47.3 ± 2.5 | 48.6 ± 2.7 | 46.8 ± 2.2 | 47.6 ± 3.6 |
Data as mol% of total fatty acids ± sd; n.d., not detectable; C1, C10, and C T3, transgenic fad5 lines expressing genomic ADS3; SALK_142406, ADS3.2 T-DNA insertion mutant.