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Expression of CRTISO in E. coli. Proteins were separated by SDS-PAGE and stained with Coomassie blue. Lane 1, Insoluble fraction (pellet) of a lysate from E. coli carrying pQE60; lane 2, soluble fraction (supernatant) of E. coli carrying pQE60; lane 3, insoluble fraction of E. coli carrying pCrtISO654; lane 4, soluble fraction of E. coli carrying pCrtISO654; lane 5, column-purified CRTISO polypeptide.
