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HPLC analysis of cis-carotene substrates and products in a CRTISO in vitro assay. A, Carotenes at the beginning of the reaction (time 0 min); B, after 90 min. Peak 1a, trans-Lycopene; peak 1b, di-cis-lycopene; peak 2, prolycopene; peak 3a, neurosporene, isomer 1; peak 3b, neurosporene, isomer 2; peak 3c, 7,9,9′-cis-isomer of neurosporene; peak 4a, 9,15,9′-cis-ζ-carotene; peak 4b, 9,9′-cis-ζ-carotene isomer; peak 5a and 5b are β-carotene isomers; peak 6, phytofluene; peak 7, phytoene.
