Table I.

Absorption spectra of carotenoids in the HPLC analysis

Peak No. in Chromatogram in Figure 3	Carotenoid	λ max nma	% III/IIab
1a	trans-Lycopene	449, 474, 503	63
1b	Di-cis-lycopene	450, 474	0
2	Prolycopene	440	0
3a	Neurosporene isomer 1	419, 440, 470	83
3b	Neurosporene isomer 2	417, 440, 469	56
3c	7,9,9′-cis-Neurosporene	411, 435, 463	0
4a	9,15,9′-cis-ζ-Carotene	(297), 380, 401, 425	76
4b	9,9′-cis-ζ-Carotene	380, 401, 425	95
5a	β-Carotene isomer 1	455, 484	13
5b	β-Carotene isomer 2	450, 476	0
6	Phytofluene	331, 350, 367	62
7	Phytoene	288	0
Standard	trans-Lycopene	449, 474, 503	62
Standard	trans-Neurosporene	420, 445, 474	90
Standard	trans-ζ-Carotene	380, 401, 425	95
Standard	trans-β-Carotene	430, 455, 484	21

^aAbsorbance spectra as detected in the HPLC running solvent: a gradient of acetonitrile:water (9:1) and ethylacetate (HPLC system 2).

^b% III/II, Fine structure of the absorption spectra expressed as the relative heights of the longest wavelength peak (III) to the middle peak (II). Zero means lack of peak III.