Figure 7.

Effects of cryptochrome mutations on light-induced expression of PpSigs, psbD, and PpRpoA genes. Protonema cells of wild-type (WT) and the cryptochrome double-knockout mutant cry1a cry1b (ΔCry) (Imaizumi et al., 2002) were grown under LL for 1 week, exposed to darkness for 24 h, and 2 h of white light (40 μmol m⁻² s⁻¹) or blue light (40 μmol m⁻² s⁻¹) or dark period was administered before the cells were sampled. Total RNAs were extracted, and the abundances of the PpSigs, psbD, and PpRpoA mRNAs were measured. Experiments were carried out as in previous Figures. The top sections show the relative levels of each mRNA. Values are means ± sd from three independent experiments. White and gray hatched bars indicate values for wild-type and cry1a cry1b cells, respectively. The bottom sections show the hybridized bands for each test gene and actin gene. A, PpSig1 mRNA; B, PpSig2 mRNA; C, PpSig5 mRNA; D, PpRpoA mRNA; E, psbD mRNA. Asterisks show genomic amplifications of PpRpoA.