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. 2004 Dec;136(4):4299–4307. doi: 10.1104/pp.104.047258

Figure 3.

Figure 3.

A, Activation of the Bplti36 promoter in response to LT (ca), exogenous ABA (aba), and drought (d) in transgenic Arabidopsis carrying the Bplti36 promoter fused to the β-glucuronidase reporter gene (uidA). Plants were transferred from 20°C to 4°C, treated by 60 μm ABA for 6 h, or exposed to drought for overnight. The effects of different treatments on the activity of Bplti36 promoter were determined by using uidA as a probe. co, Control plants grown at normal growth conditions. B, Activation of the Bplti36 promoter in CBF3 overproducers (crCBF3) and corresponding vector controls (crB6) in normal-growth conditions (c) and after exposure to cold (ca; 4°C) for 6 h. Transgenic Arabidopsis carrying Bplti36 promoter fused to uidA-reporter gene were crossed with CBF3 overproducer (crCBF) and corresponding-vector control B6 (crB6). The activity of Bplti36 promoter in crosses were determined by using uidA as a probe. The efficacy of the cold treatment and equal RNA loading were controlled with an LTI78 probe and total RNA staining with methyl blue staining, respectively.