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. 2004 Dec;136(4):4299–4307. doi: 10.1104/pp.104.047258

Table I.

The effect of photoperiod and temperature on freezing tolerance of birch leaves

Condition Cont 1 d 2 d 4 d 7 d 14 d
LT50
SD −2.5 ± 0.21 −3.4 ± 0.25 −4.5 ± 0.31 −5.9 ± 0.28 −6.4 ± 0.35 −7.1 ± 0.6
LT −2.5 ± 0.21 −4.7 ± 0.28 −6.8 ± 0.54 −10.5 ± 0.47 −11.6 ± 0.53
SD + LT −6.4 ± 0.35 −9.6 ± 0.65 −12.0 ± 0.73 −14.8 ± 0.66 −15.8 ± 0.84

Birch seedlings were exposed to SD length (12 h light) for 7 d; LT in LD condition (4°C, 24 h light) for 7 d and SD for 7 d followed by LT (4°C) for 7 d. The freezing tolerance (LT50) of leaves (means ± se) was measured by ion-leakage test (Sukumaran and Weiser, 1972).