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Localization of the ropporin 1 transcript in human sperm nucleus. A) Localization in human sperm nucleus partially decondensed in the presence of DTT and Heparin. B) Localization in sperm halo preparations using 10 mM DTT and 2M NaCl to extract the protamines and histones respectively. The specificity of the signal for RNA was verified on decondensed nuclei and halo preparations treated with DNAse 1 (signal present) and RNAse (signal absent) before in situ hybridization.
