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. 2017 Jan 6;6(1):40–55. doi: 10.1080/21623945.2016.1277052

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© 2017 The Author(s). Published with license by Taylor & Francis

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 1. — Overexpression of hTERT and HPV16-E7 in _imAPAD and _imGPAD cell lines. (A) overexpression of hTERT and HPV16-E7 protein was confirmed by Western blotting in the paired _imAPAD and _imGPAD cell lines (passage 15–17) and compared with 1°APAD and 1°GPAD preadipocytes (passage 6) from the same donor. Labeling for actin is shown as a loading control. (B) Telomerase activity was determined in _imAPAD and _imGPAD cell lines (passage 11) and 1°APAD and 1°GPAD cells (passage 6) (n = 3, mean ± SEM; *P < 0.05, paired samples t-test). Heat treatment was used to inactivate telomerase as a negative control.