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. 2017 Jan 6;6(1):40–55. doi: 10.1080/21623945.2016.1277052

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© 2017 The Author(s). Published with license by Taylor & Francis

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 2. — Proliferation of _imAPAD and _imGPAD cell lines. (A) Light microscopy of proliferating _imAPAD and _imGPAD cell lines compared with 1°APAD and 1°GPAD cells (x 100 magnification). (B) Cell doubling time of paired _imAPAD/_imGPAD cell lines was compared with 1°APAD/1°GPAD cells (passage 9–12). Proliferation rates were examined up to passage 30 for _imAPAD/_imGPAD cells but 1°APAD and 1°GPAD cells failed to proliferate after passage 14 (n = 6–8, mean ± SEM; ***P < 0.001; paired samples t-test for cell line vs. primary cells).