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. 2017 Jan 6;6(1):40–55. doi: 10.1080/21623945.2016.1277052

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© 2017 The Author(s). Published with license by Taylor & Francis

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 7. — Lipolysis in _imAPAD and _imGPAD cell lines. (A) Basal glycerol release plotted against total TAG content in _imAPAD and _imGPAD cell lines (passage 16–17). (B) Catecholamine-stimulated glycerol release in _imAPAD and _imGPAD cells relative to basal glycerol release. Cells were treated with 5 mM KRH buffer containing either adrenaline (100nM) or isoprenaline (100nM) (n = 3, mean ± SEM, normalized for total TAG content; *P < 0.05, Wilcoxon signed-rank). (C) mRNA expression of adrenergic receptors and lipases at day 14 of adipogenic differentiation in _imAPAD and _imGPAD cells (passage 8–9). Data are shown as ΔΔCt values (normalized to PPIA and PGK1; n = 6, mean ± SEM).