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. 2017 Jan 6;6(1):40–55. doi: 10.1080/21623945.2016.1277052

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© 2017 The Author(s). Published with license by Taylor & Francis

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 8. — Inherent depot-specific expression of developmental genes in _imAPAD and _imGPAD cells. mRNA expression of HOXA5 and HOTAIR over a 14 day adipogenic differentiation time-course was determined by real-time qPCR in _imAPAD and _imGPAD cell lines (A and B) and 1°APAD and 1°GPAD cells (C and D). Data are shown as ΔΔCt (normalized to PPIA and PGK1; n = 6, mean ± SEM). A multivariate general linear model was used to test for statistical significance between depots and time, and to assess depot x time interactions. P-values are presented in the shaded boxes, NS: non-significant.