Gene Expression Analysis of FRA3 by Semiquantitative RT-PCR.
(A) Expression of the FRA3 gene in various Arabidopsis organs. The expression level of a ubiquitin gene was used as an internal control. The seedlings used were 2 weeks old. Mature leaves were from 6-week-old plants. Flowers and mature roots were from 8-week-old plants. Stems I and II were from 4- and 8-week-old plants, respectively.
(B) Expression of the FRA3 gene in seedlings of the wild type, fra3 mutants, and a representive fra3 complementation line (fra3 compl.).
(C) Overexpression of wild-type FRA3 cDNA (35S::FRA3) or mutant fra3 cDNA (35S::fra3) in wild-type seedlings under the control of the 35S promoter of Cauliflower mosaic virus. Shown are four representative lines of 35S::FRA3 and three representative lines of 35S::fra3.