Fig 2.
Time-killing curves of SHa and its analog [K3]SHa against S. aureus ST1065 (A) and E. coli ML-35p (B). Bacteria (106 cfu/ml) were incubated in phosphate-buffered saline (PBS) with temporins at concentrations 2-fold above the MIC obtained for S. aureus ST1065 (6 μM for [K3]SHa and 12 μM for SHa). The negative control corresponds to bacteria incubated in PBS without peptide (w/o peptide). The data are shown as the means ± SEM from a single experiment carried out in triplicate and are representative of three independent experiments.