Fig 5.
Temporin-induced SYTOX Green (SG) influx into the bacteria K. pneumoniae ATCC 13883 (A) and S. pyogenes ATCC 19615 (B), and the parasites L. infantum (C), L. braziliensis (D), L. major (E), and T. cruzi (F). Bacteria (106 cfu/ml) and parasites (2.5 x 106 cells/ml) were preincubated with 1 μM SG, and peptides (SHa or [K3]SHa) were added after fluorescence stabilization. Membrane alteration is correlated with the fluorescence of the DNA fluorescent probe (λexcitation = 485 nm and λemission = 520 nm). For bacteria, the data are expressed as the means ± SEM after subtraction of the negative control values (w/o peptide) from the test values. For parasites, the results (mean ± SEM) were plotted as a percentage of the fluorescence relative to that of parasites fully permeabilized by 0.1% Triton X-100. The curves correspond to one experiment carried out in triplicate and are representative of two independent experiments.