Fig 7. Changes in the membrane potential of bacteria and parasites upon addition of temporins.
S. aureus ATCC 25923 (A), L. infantum (B), L. amazonensis (C) and T. cruzi (D) were equilibrated with DiSC3(5) (1 μM for S. aureus and 2.5 μM for parasites). SHa or [K3]SHa was then added (t = 0) at a concentration of 5 μM (bacteria) or 50 μM (parasites), and changes in the fluorescence were monitored for 15 min (bacteria) or 20 min (parasites) at λexcitation = 622 nm and λemission = 670 nm. The curves correspond to a single experiment representative of three independent experiments.