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. 2017 Mar 20;12(3):e0174024. doi: 10.1371/journal.pone.0174024

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© 2017 Raja et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 8 — AFM and FEG-SEM visualization of the effect of temporins-SH on P. aeruginosa bacteria (A–C) and parasites (L. infantum promastigotes and T. cruzi epimastigotes; D–I). A–C, AFM imaging of P. aeruginosa: A, untreated control bacteria; B, bacteria after 1 h incubation with 50 μM SHa; C, bacteria treated for 1 h with 6 μM [K³]SHa. Bacteria were severely damaged by temporins compared to the control. D–G, AFM imaging of L. infantum promastigotes and T. cruzi epimastigotes: D and E, L. infantum untreated (D) or treated with 5 μM [K³]SHa (E); F and G, T. cruzi without peptide (F) or with 5 μM [K³]SHa (G). H and I, FEG-SEM visualization of L. infantum promastigotes without peptide (H) or with 5 μM [K³]SHa. Morphological changes were observed for parasites that were incubated with peptides (E, G and I).