Table 3. Antibacterial activity of temporin-SHa analogs compared to the parent peptide.
| MIC a, MICGMb (μM) | ||||
|---|---|---|---|---|
| SHa | [K3]SHa | [A2,6,9]SHa | [A2,6,9, K3]SHa | |
| Gram-negative bacteria | ||||
| E. coli ATCC 25922 | 10 | 3 | > 200 | > 200 |
| E. coli ML-35p | 10 | 3 | > 200 | > 200 |
| P. aeruginosa ATCC 27853 | 50 | 6 | > 200 | > 200 |
| S. entericac | 25 | 6 | > 200 | > 200 |
| A. baumannii ATCC 19606 | 6 | 3 | > 200 | > 200 |
| K. pneumoniae ATCC 13883 | 12 | 3 | > 200 | > 200 |
| 14.4 | 3.8 | - | - | |
| Gram-positive bacteria | ||||
| S. aureus ATCC 25923 | 3 | 3 | > 200 | > 200 |
| S. aureus ST1065 | 6 | 3 | > 200 | > 200 |
| S. aureus ATCC 43300d | 6 | 3 | - | - |
| S. aureus ATCC BAA-44e | 6 | 3 | - | - |
| S. pyogenes ATCC 19615 | 6 | 1 | > 200 | 100 |
| L. ivanovii | 6 | 3 | > 200 | > 200 |
| E. faecalis ATCC 29212 | 12 | 6 | > 200 | > 200 |
| 6 | 2.8 | - | - | |
| Yeasts/fungi | ||||
| C. albicans ATCC 90028 | 25 | 6 | > 200 | > 200 |
| C. parapsilosis ATCC 22019 | 100 | 25 | > 200 | > 200 |
| S. cerevisiae | 12 | 3 | > 200 | > 200 |
| 31.1 | 7.7 | - | - | |
a Values represent the means of three independent experiments performed in triplicate.
b MICGM denotes the geometric mean of the MIC values (indicated in bold) for all Gram-negative, Gram-positive and yeast/fungal strains.
c Serotype Enteritidis.
d Resistant to methicillin and oxacillin.
e Resistant to amoxicillin/clavulanic acid, cephalothin, ciprofloxacin, erythromycin, gentamicin, imipenem, oxacillin, penicillin, tetracycline, ampicillin, doxycycline, methicillin, azithromycin, ceftriaxone, clindamycin, lincomycin, perfloxacin, rifampin, and tobramycin.