Table 5. Cytotoxic activity of temporins SHa and analogs against human cells and rat erythrocytes.
| IC50 or LC50 (μM) | ||||
|---|---|---|---|---|
| Cells | SHa | [K3]SHa | [A2,6,9]SHa | [A2,6,9, K3]SHa |
| Rat erythrocytes | 25 | 26 | > 200 | > 200 |
| Human erythrocytes | 25 | 50 | > 100 | ND |
| Human THP-1 monocytes | > 60 | 48 | > 60 | ND |
| Human THP-1-derived macrophages | 60 | 55 | ND | ND |
| Human HepG2 | 560 | 358 | > 600 | ND |
| Human fibroblasts | > 100 | > 100 | ND | ND |
Values were determined with GraphPad Prism 6.0 software and are the means of three independent experiments performed in triplicate. IC50, half maximal inhibitory concentration; LC50, half maximal lytic concentration (erythrocytes and macrophages).
ND: not determined.