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. 2017 Mar 20;12(3):e0174125. doi: 10.1371/journal.pone.0174125

Fig 3. Expression and purification of WT- (A) and C105A- (B) calpain by conventional and AF methods.

Fig 3

NI: Non-induced; I: Induced; DEAE, MonoQ: anion exchange chromatography using diethylaminoethyl and MonoQ column; Histrap: immobilized metal affinity chromatography; GF: gel filtration; AF: immobilized b-hCSD1 affinity chromatography.