Table 1. Comparison of the kinetic parameters of the enzyme purified by two methods.

	Km (μM)	Vmax (nM.s-1)	Kcat(s-1.10−3)	Kcat/Km (μM-1.s-1. 10−3)	Specific Activity (nmol.s-1.mg-1)
AF	29.32 ± 5.66	1.708 ± 0.233	12 ± 1	41.3 ± 2.6	0.112 ± 0.014
Conventional method	20.54 ± 2.28	2.262 ± 0.209	15 ± 2	73.2 ± 4.6	0.148 ± 0.015