Figure 1.

Fractionation of Telomerase Complexes by Gel Filtration.

(A) Tobacco BY-2 cell extracts were fractionated by gel filtration and the concentration of protein for each fraction was determined. Numbers at the top of the figure indicate the sizes of molecular mass markers in kD (thyroglobulin, 669 kD; ferritin, 440 kD; catalase, 232 kD).

(B) Telomerase activity elutes as a major 600-kD complex and an additional lower molecular weight complex (250 kD). After gel filtration and concentration of proteins, telomerase activity was evaluated by the TRAP assay.