Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2004 Dec;16(12):3370–3385. doi: 10.1105/tpc.104.026278

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2004, American Society of Plant Biologists

PMC Copyright notice

Figure 7. — Nuclear Staining and in Situ Detection of DNA Fragmentation in Wild-Type and Transgenic Tobacco BY-2 Cells at Different Stages of Subculture.

(A) Three-day-old wild-type, 35S::GUS, 35S::NgTRF1, and 35S::anti-NgTRF1 transgenic cells in the 35th round of batch subculture were stained with DAPI (panel a) and assayed with the TUNEL technique (panel b) as described in Methods to detect nuclei and fragmented nuclear DNA, respectively. Panel c shows a magnified view of the boxed portion in panels a and b. Bars = 25 μm in panels a and b; bars = 100 μm in panel c.

(B) Twelve-day-old wild-type, 35S::GUS, 35S::NgTRF1, and 35S::anti-NgTRF1 transgenic cells in the 35th round of batch subculture were stained with DAPI (panel a) and assayed with the TUNEL technique (panel b). Panel c shows a magnified view of the boxed portion in panels a and b. Bars = 25 μm in panels a and b; bars = 100 μm in panel c.