Table 1.
Percentage of Apoptotic Cells of Tobacco BY-2 Cells in the 35th Round of Subculture
Days in Subculture | Cell Line | Total Cell No. Counted | Apoptotic Cell No. | Ratio |
---|---|---|---|---|
Three-day-old cells | Wild type | 1950 | 4 | 0.02% |
35S::GUS | 1840 | 5 | 0.02% | |
35S::NgTRF1-a | 1752 | 211 | 12.0% | |
35S::NgTRF1-c | 1356 | 129 | 9.5% | |
35S::Anti-NgTRF1-d | 1950 | 89 | 4.6% | |
35S::Anti-NgTRF1-f | 1940 | 80 | 4.1% | |
Twelve-day-old cells | Wild type | 1900 | 44 | 2.3% |
35S::GUS | 1840 | 37 | 2.0% | |
35S::NgTRF1-a | 1645 | 291 | 17.6% | |
35S::NgTRF1-c | 1657 | 232 | 14.0% | |
35S::Anti-NgTRF1-d | 1570 | 115 | 7.3% | |
35S::Anti-NgTRF1-f | 1640 | 85 | 5.2% |