Figure 2.

Residue-specific differences of NMR ¹⁵H backbone transverse relaxation rates of 300 μM (a) WT p53TAD (red solid circles) and (b) K24N mutant (blue solid circles) in the presence (2.5 μM) and absence of SNPs, i.e. $\mathrm{\Delta }{R}_{2,i}=R_{2,i}^{\text{SNP}}-R_{2,i}^f$, were plotted as a function of residue number. (a),(b) Predicted ΔR₂ values by the free residue interaction model (FRIM) (Eq. (5)) are shown as black solid circles (except black open circles for Pro residues). (c) Difference of experimental ΔR₂ profiles of (a) and (b) (green squares), ΔΔR₂, depicting the effect of Lys24 (black arrows) on ΔR₂. The dashed orange line is a fit using Eq. (4). (d) Cumulative effect of positively charged residues on global ΔR₂ profiles using Eq. (4) for WT (red) and K24N mutant (blue).