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. 2017 Mar 20;12(3):e0173713. doi: 10.1371/journal.pone.0173713

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© 2017 Ota et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 1 — (A) Conditional gene targeting of the murine Utx locus. (B) Immunoblotting for Utx and β-actin in Utx-deficient and control mESCs. A representative result from three independent mESC clones is shown. Band densities of Utx and β-actin were quantified by ImageQuant TL ver. 8.1 and normalized to β-actin. The data are presented relative to the signal of control mESCs. (C) Immunoblotting for H3, H3K4me3, H3K9me3, H3K27me3, and H3K36me3 in mESCs. A representative result from three independent mESC clones is shown. Band density of histones was quantified by ImageQuant TL ver. 8.1 (GE healthcare life sciences, Pittsburgh, PA, USA), and data are shown by the relative value of histone methylations normalized to H3.