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. 2017 Mar 20;12(3):e0174017. doi: 10.1371/journal.pone.0174017

Fig 4. HNF1α activates the NF-κB signaling.

Fig 4

(A) Huh7 cells were co-transfected with pNF-κB-luc (0.2 μg), pRL-TK (0.1 μg) and pCMV-HNF1α (0.2 μg) or pCMV-p65 (0.2 μg). Means and SEMs of relative luciferase activity data are plotted, with the means of the values from pCMV-transfected cells (control) taken as 1. ** P < 0.01, *** P < 0.001. (B) Huh7 cells were co-transfection with pCMV-HNF1α 0.1, 0.2, 0.4, 0.8, or 1.0 μg. Cells were lysed and separated into cytoplasmic and nuclear fractions. Histone 3 served as a loading control of nuclear fraction samples.