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. 2017 Mar 10;23:45–57. doi: 10.12659/MSMBR.901462

Figure 6.

Figure 6

IKCa1 siRNAs downregulates IKCa1 mRNA and protein expression in HeLa cells. (A, B) Effect of the IKCa1-specific siRNAs on IKCa1 mRNA and protein expression levels, respectively. “nc” represents cells transfected with pGensil-HK+Lipo. “sr1” represents that HeLa cells were transfected with pGenesil-IKCa1siRNA+ Lipo and “sr2” represents a repeat of this experiment. The letter b (blank) represents cells were transfected with RPMI-1640+Lipo. At 48 h post-transfection, mRNA and protein levels were quantified and normalized to housekeeping genes GAPDH and β-actin, respectively. Data are the mean values ±SE of 3–5 independent transfections. * p<0.05, vs. non-transfected (blank) cells as determined by t test.