(top) Per-residue backbone
RMSF; blue, green, and red plots correspond to increasing ionic strength,
respectively. (middle) RMSF mapped onto six subunits of a 24-mer assembly
encompassing 4-fold (blue stars), 3-fold (green stars), and B-pores
(red stars) shows that the flexibility near 4-fold and B-pores in
wt BfrB is dampened in the mutants. In the color scale, flexibility
increases from white to red. (bottom) The average conformation explored
by FC residues during MD simulations is shown as red mesh; the gate
open (green) and gate closed (cyan) conformations of H130 are shown
in sticks.