FIG 4 .

Detailed analysis of D. acidovorans sequence variation indicates the presence of subpopulations that are evolving in situ. (A) Shown are the locations of all of the differences between the contaminant D. acidovorans ANG1 genome recovered in this study and the D. acidovorans SPH-1 genome, as well as the alignment of the assembled contigs and coverage of the genome. (B) The frequencies of specific SNPs in samples sequenced at different time points exhibit three distinct patterns suggestive of three subpopulations (cohorts). Abundances fluctuate, but there is an overall progression toward fixation.