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. 2017 Mar 19;19(4):364–373. doi: 10.1016/j.neo.2017.02.009

Figure 1.

Figure 1

In vitro effects of RTV, IDV, and PHZ on glioblastoma cells. (A) IC50 values determined for IDV, PHZ, and RTV in U87MG cells. IC50 represents the drug concentration reducing by 50% the growth of treated cells compared with control ones. The IC50 of RTV was 45 μM, approximately 10 times lower than the IC50 of IDV (500 μM). We were unable to determine the IC50 of PHZ because PHZ never induced 50% inhibition compared with control cells even at the maximal concentration we could dissolve PHZ in DMSO (100 mM). Y-axis (T/NT) represents the mean value of the ratio between the number of the cells in the wells (at least 3) with the addition of the drug (T) and the number of the cells in the control wells (NT) (at least 3). (B-G) Growth curves derived from RTV treatment of the human U87MG glioblastoma cell line (B), GBM-P1 primary human glioblastoma-derived cells (C), human glioblastoma Hu197 cell line (D), and mouse glioblastoma GL261 cell line (E). Growth response of U87MG glioblastoma cell line to the addition of IDV (F) or PHZ (G) was also tested in vitro. The growth was followed up to 72 hours. RTV and IDV were used at the IC50 concentration we previously determined for each cell type, whereas PHZ was used at the maximal concentration we tested before. For RTV, IC50 was 45 μM for U87MG, GBM-P1, and Hu197 and 55 μM for mouse GL261 cells. RTV induced consistent growth inhibition in all glioblastoma cell cultures. Error bars depict standard deviation.