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. Author manuscript; available in PMC: 2018 Mar 15.
Published in final edited form as: Cancer Res. 2017 Feb 15;77(6):1492–1502. doi: 10.1158/0008-5472.CAN-16-2755

Fig.4. Aberrant Syk activation induces MCP-1 overexpression via Stat3 signaling in TSC2-deficient cells.

Fig.4

(A) TSC2- and TSC2+ cells were treated with either control vehicle (DMSO) or R406 (1μM) for 24 h. (B) TSC2- cells were transfected with control siRNA (Ct) or Syk siRNA for 48 h. (A and B) Levels of MCP-1 in cell culture supernatants were determined by enzyme-linked immunosorbent assay (ELISA). Data represent means ± SEM of three independent experiments. ***P < 0.001 (versus TSC2- cells treated with DMSO), by one-way ANOVA (A); * P < 0.05, by Student’s t test (B). (C) TSC2- and TSC2+ cells were treated with either control vehicle (DMSO) or R406 (1μM) for 24 h. (D) TSC2- cells were transfected with control siRNA (Ct) or Stat3 siRNA for 48 h. (C and D) Equal amounts of protein from whole cell lysates were analyzed by Western blot using antibodies against Phospho-Stat3 and Stat3. β-actin was used as a loading control. (E) TSC2- cells were transfected with control siRNA (Ct) or Stat3 siRNA for 48 h. Levels of MCP-1 in cell culture supernatants were determined by ELISA. Data represent means ± SEM of three independent experiments. * P < 0.05, by Student’s t test.