Fig 1. H77/JFH1_ΔHVR1 escaped from HMAb AR5A antibody neutralization.

(A) Huh7.5 cells were infected with H77/JFH1_ΔHVR1 and treated with 5 μg/ml of AR5A antibody during the indicated period of time. Treatment I, II, III were treated independently and cultured along with the untreated control. (B) 1st passage of virus supernatant taken from treatment I and untreated control were subjected to a ten-fold dilution series of AR5A starting at 50 μg/ml. The virus/antibody mixes along with virus only were added to Huh7.5 cells for 4 hour prior to wash and addition of fresh medium. Following a total of 48 hour infection the cells were immunostained and the number of FFUs per well were counted as described in Materials and Methods. Neutralization data are shown as the mean of four replicates. Neutralization was related to infection of 8 virus replicates in the absence of antibody. Three-parameter curve-fitting was used to obtain sigmoidal dose-response curves. Error bars represents the standard errors of the mean.