Table 1.
Plasmids.
| Plasmid | Description | Marker | Source |
|---|---|---|---|
| pCS623 | KAR2 | CEN URA3 | (Wang et al., 2014) |
| pCS757 | KAR2-FLAG | CEN LEU2 | (Wang et al., 2014) |
| pCS878 | kar2-K314E-FLAG | CEN LEU2 | This study |
| pCS452 | PGAL1-ERO1*-myc | CEN URA3 | (Sevier et al., 2007) |
| pCS681 | KAR2 | CEN LEU2 | (Wang et al., 2014) |
| pCS685 | kar2-C63A | CEN LEU2 | (Wang et al., 2014) |
| pKP37 | kar2-K314E | CEN LEU2 | This study |
| pKP97 | kar2-C63A-K314E | CEN LEU2 | This study |
| pJW7 | SIL1 | CEN URA3 | This study |
| pCS876 | sil1-C52A-C57A | CEN URA3 | This study |
| pHS116 | SIL1 | CEN LEU2 | This study |
| pKS20 | sil1-C52A | CEN LEU2 | This study |
| pKS21 | sil1-C57A | CEN LEU2 | This study |
| pKS24 | sil1-C52A-C57A | CEN LEU2 | This study |
| pKS23 | sil1-C203A | CEN LEU2 | This study |
| pKS22 | sil1-C373A | CEN LEU2 | This study |
| pCS923 | sil1-C52A-C57A-C203A-C373A | CEN LEU2 | This study |
| pCS925 | sil1-H163E | CEN LEU2 | This study |
| pCS817 | His6-kar2-(42-682) | KAN | (Wang et al., 2014) |
| pCS818 | His6-kar2-(42-682)-C63A | KAN | (Wang et al., 2014) |
| pKP85 | His6-kar2-(42-682)-K314E | KAN | This study |
| pCS637 | sil1-(20-407)-His6 | AMP | This study |
| pCS870 | sil1-(20-407)-C52A-His6 | AMP | This study |
| pCS871 | sil1-(20-407)-C57A-His6 | AMP | This study |
| pCS875 | sil1-(20-407)-H163E-His6 | AMP | This study |
| pCS877 | sil1-(20-407)-C52A-C57A-His6 | AMP | This study |
| pCS948 | sil1-(20-407)-C203A-C373A-His6 | AMP | This study |
| pCS895 | sil1-(20-407)-C52A-C57A-C203A-C373A- His6 | AMP | This study |
| pKP52 | GST-sil1-(22-406) | AMP | This study |
| pHS130 | His6-lhs1-(21-877)-StrepII | KAN | (Xu et al., 2016) |
| pCS675 | GST-sec63J-(121-221) | AMP | (Wang et al., 2014) |