Table 1.

Candidates selected for verification in mammalian cells.

DOI: http://dx.doi.org/10.7554/eLife.23816.012

Yeast	Human	Description*
NVJ2 (YPR091C)	HT008	Lipid-binding ER protein, enriched at nucleus-vacuolar junctions; may be involved in sterol metabolism or signaling.
ERG26	NSDHL	ERGosterol biosynthesis, catalyzes the second of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis.
ERG9	FDFT1	ERGosterol biosynthesis, squalene synthase; joins two farnesyl pyrophosphate moieties to form squalene in the sterol biosynthesis pathway.
SCS2	VAPA	Integral ER membrane protein, regulates phospholipid metabolism; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to plasma membrane (PM) and regulate PI4P levels.
DPM1	DPM1	Dolichol phosphate mannose synthase of ER membrane; required for biosynthesis of glycosyl phosphatidylinositol (GPI) membrane anchor, as well as O-mannosylation and protein N- and O-linked glycosylation.
EMC2	EMC2	Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER.

*From the Saccharomyces Genome Database (SGD).