Skip to main content
. Author manuscript; available in PMC: 2018 Jan 1.
Published in final edited form as: Exp Eye Res. 2016 Nov 17;154:177–189. doi: 10.1016/j.exer.2016.11.013

Figure 6. RGC precursors express CD184 prior to CD171.

Figure 6

A) On day 31 and day 34 of differentiation 15 retinal organoids were labeled with 2 μM EdU for 10 hours followed by a 4 day or 14 hour chase, respectively. On day 35, following dissociation, retinal organoid cells were stained with anti-CD184-APC and anti-CD171-PE then live cells sorted using the gates shown. B) EdU+ cells in sorted populations detected by Click-iT chemistry are shown as the average +/− SD EdU+ cells on 2 coverslips. The total number of cells analyzed to generate data is indicated in the respective columns. C) Immunodetection of BRN3+ cells in the sorted populations. The number of cells analyzed to generate data in columns from left to right are indicated. D) The EdU+ and BRN3+ cell data (B and C) was used to calculate the fraction of BRN3+ cells labeled by EdU.