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. Author manuscript; available in PMC: 2017 Nov 30.
Published in final edited form as: Mol Psychiatry. 2016 Sep 20;22(12):1714–1724. doi: 10.1038/mp.2016.155

Figure 5. Decreasing PDE11A expression increases IL-6 expression.

Figure 5

To determine if decreased PDE11A4 may drive expression of pathophysiological markers, we examined expression of the proinflammatory cytokine IL-6. A) Western blots show that B) C57BL/6J mice express more IL-6 relative to BALB/cJ mice in both the cytosolic and membrane fractions of DHIPP (n=6/strain for membrane and 8/strain for cytosol). C) In VHIPP, however, C57BL/6J mice only express more IL-6 relative to BALB/cJ mice in the cytosolic fraction (n=8/strain/compartment). D) Interestingly, PDE11A4 expression in the VHIPP membrane fraction correlates negatively with IL-6 expression in the VHIPP cytosol, such that C57BL/6J and BALB/cJ mice with lower PDE11A4 expression exhibit higher levels of IL-6 expression. E) Decreasing PDE11A expression appears sufficient to upregulate IL-6 because PDE11A KO mice demonstrate significantly more IL-6 expression relative to paired sex-matched WT littermates in DHIPP (WT, n=17; KO, n=19). Data in panel B passed normality and equal variance. Data in panel C and E failed normality; therefore, nonparametric tests were used for those datasets. Data are graphed as means ±SEM. Brightness and contrast adjusted for graphical clarity of blot images. Post hoc, *vs. BALB/cJ or WT, P≤0.005-0.001.