Skip to main content
. Author manuscript; available in PMC: 2018 Mar 1.
Published in final edited form as: Eur J Neurosci. 2017 Feb 15;45(6):826–836. doi: 10.1111/ejn.13528

Figure 1.

Figure 1

Effect of chronic food restriction (FR) on AMPAR subunit expression and distribution in nucleus accumbens (NAc) as assessed by BS3 cross-linking studies. A and C: Quantification of monomeric AMPAR proteins, normalized to α-tubulin, revealed an increase in uncrosslinked (−BS3) monomeric GluA1 (A) but not GluA2 (C) with no change following BS3 cross-linking (+BS3) when compared to ad libitum (AL) fed control. B and D: Shown are representative immunoblots depicting the levels of uncrosslinked, monomeric AMPARs (total) while cross-linked AMPARs were observed as both high-molecular weight bands of 300- 600kDA (surface) and unmodified, monomeric bands of 100kDA (intracellular). Data obtained from individual subjects are expressed in comparison to the normalized control and displayed with mean ± s.e.m, n=9 per group, **p<.01.