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. 2004 Dec 15;18(24):3004–3009. doi: 10.1101/gad.1255404

Figure 2.

Figure 2.

AAK-2 has kinase activity and functions to extend lifespan. (A) Phylogenetic analysis of the AAK-1 and AAK-2 sequences. Additional sequences used were hAMPKα1 (GI:20178277), hAMPKα2 (GI:20178276), DmSNF1A (GI:17137472), AtSNF1 (GI:30678280), and ScSNF1 (GI:6320685). Bar is 0.1 substitutions per site. (B) Survival curves of animals with decreased, normal, or increased aak-2 gene dosage. For statistical data see Supplementary Table 1. (C) Representative pictures and quantification of fluorescence in the anterior intestine as a function of adult age and genotype (400-msec exposures; bar is 50 μm; anterior is left, dorsal is up). ★P < 0.0001; otherwise P > 0.05 (unpaired t-test). (D,E) Measurements of kinase activity in AAK-2-GFP immunoprecipitates using the SAMS peptide assay. (D) Increasing the AMP concentration leads to an increase in kinase activity. (E) Treatment with protein phosphatase-1 greatly reduces kinase activity. ★P < 0.0001 compared with no treatment (unpaired t-test).